Annual Riport of the Laboratory of Biocusmicalgenetics National Heart and Lung

Fusion of clonal neuroblastoma cells with rat glioma cells yielded clonal hybrid cell lines which synthesize, store and excrete acetylcholine; properties which are not expressed by the parental cell lines. Cells from one hybrid line were found to form synapses with cultured striated muscle cells. Synapses between hybrid cells ar&muscle cells closely resemble the synapses between normal motor neurons an&striated muscle before they are fully developed. Underappropriatie con&t-. hybrid cells establish synaptic connections with virtually every muscle.cell tested; thus,.synaptic connections are formed in abundanceL.:.Martted differences were observed in the efficiency of transmission across diffekeat syrt#e&.:~Axomal activities~whkch were foyad to be regulated include--choline &etyltramsferase,,. acetylcholinesterase, Na action potential ionophos&s@ecific ac&itiea,.and.thc rate.oE.choline.transport into cells.